| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239634 |
| Chromosome: | chromosome 2 |
| Location: | 3393350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095104 | (1 of 1) K15193 - protein SPT2 (SPTY2D1, SPT2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATTGAAGCGGTGGCAAATACACTCG |
| Internal bar code: | GGCTCCAAGATCAAAGTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 98.6453 |
| LEAP-Seq n confirming: | 5534 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTAATCAGAAGCCCCC |
| Suggested primer 2: | CCTGTGAGACACGATAGCGA |