| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239646 |
| Chromosome: | chromosome 13 |
| Location: | 4865231 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605650 | ALDH10,ALDH10A1,ALD7 | (1 of 1) 1.2.1.8 - Betaine-aldehyde dehydrogenase / Betaine aldehyde oxidase; Aldehyde dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAAGGGTGGAGAGGCGGTGTGAGGCGC |
| Internal bar code: | AAGAGCTGTGTGACAGAGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCGCGTAGTAGTCGAAG |
| Suggested primer 2: | CGTGTTATTTGGGCTCCACT |