| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239650 |
| Chromosome: | chromosome 1 |
| Location: | 53730 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000250 | SNR1 | (1 of 2) PTHR12677:SF5 - TVP38/TMEM64 FAMILY MEMBRANE PROTEIN YDJX; Predicted SNARE-associated Golgi protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAAAATGTGAAGAACGAGGTAGAATCT |
| Internal bar code: | GTGTGTCGTCACGGGCTTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 842 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTACGGTACTGCCACCCC |
| Suggested primer 2: | GATGGAAGCAACTAGCGAGG |