| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239654 |
| Chromosome: | chromosome 13 |
| Location: | 3725934 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589300 | CND2 | (1 of 1) PF05786 - Condensin complex subunit 2 (Cnd2); Condensin complex component | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCGGGGCTGGCGGCGGCACTGGCGGGG |
| Internal bar code: | CTCGTGTGCGGATGTTATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATCTCCTCCATTGCAT |
| Suggested primer 2: | ACATCGTATTTGACGCCTCC |