| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239661 |
| Chromosome: | chromosome 12 |
| Location: | 4392551 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520550 | TRN1 | (1 of 1) K18752 - transportin-1 (TNPO1); Importin beta | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGCACCGTTTTGCCCCAGGAGACAGG |
| Internal bar code: | TGAAGGTGACTGTTCAATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 99.5553 |
| LEAP-Seq n confirming: | 7164 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTTGGGTTCAGGTGGAG |
| Suggested primer 2: | ATACTGTATCCGCACCCTCG |