| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239667 |
| Chromosome: | chromosome 17 |
| Location: | 3675521 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726750 | SHK1,SHKA1,DHAPS1 | (1 of 1) 2.5.1.54 - 3-deoxy-7-phosphoheptulonate synthase / Phospho-2-oxo-3-deoxyheptonate aldolase; DHAP synthetase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACATGCTCAATGTCCAAGCCACGCACGC |
| Internal bar code: | GCGAAATGAAAGGTCCAGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 99.6853 |
| LEAP-Seq n confirming: | 9504 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGGTTAGGGGAATAGA |
| Suggested primer 2: | TGAGCAAGAGGGTGTGAGTG |