| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239686 |
| Chromosome: | chromosome 16 |
| Location: | 5583246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679950 | RFC3 | DNA replication factor C complex subunit 3; (1 of 1) PTHR11669:SF1 - REPLICATION FACTOR C SUBUNIT 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGGGCCGAGAGCAAGCACAACCTGGAC |
| Internal bar code: | TTTTCCATTCTTGCTCTAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.3239 |
| LEAP-Seq n confirming: | 3526 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGACAGGGTTTAGACAGG |
| Suggested primer 2: | GGCTACAGGGGTGTAGGTCA |