Insertion junction: LMJ.RY0402.239710_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.239710
Chromosome:chromosome 6
Location:7791119
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g302450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCCAGGCGCGCGAGCTCTCCCCATCACC
Internal bar code:CGCCAGAAGACGTCTTAGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:620
LEAP-Seq percent confirming:98.6164
LEAP-Seq n confirming:1568
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCAACCTCAACACCTTCC
Suggested primer 2:ACACGCAACATGTCGGATAA