Insertion junction: LMJ.RY0402.239721_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.239721
Chromosome:chromosome 9
Location:3844019
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g391875 (1 of 1) PTHR22847//PTHR22847:SF453 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGCCGGGCCACAGTCACGCCTGCGGGG
Internal bar code:TAGGAAAGATTTTTTGCACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:58
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTGTATACGCTTCCTTG
Suggested primer 2:ACTCCAGCTATCTCGCCAAA