| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239765 |
| Chromosome: | chromosome 6 |
| Location: | 7402520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299550 | ZIP6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGTGGCGTGGTGTGCGTGCGGCGTGT |
| Internal bar code: | CCATTGGGTAACTGGGGTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 99.0156 |
| LEAP-Seq n confirming: | 1207 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTAACCCGCATTCCAAAC |
| Suggested primer 2: | AATGGCGTGTGTATCGTTCA |