| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239792 |
| Chromosome: | chromosome 2 |
| Location: | 3336390 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095092 | PSY1 | Phytoene synthase; (1 of 1) K02291 - phytoene synthase (crtB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGGGGAAACATTGCTGGCAGCCAGCAC |
| Internal bar code: | CGACACCCTGGTATACAAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 99.7988 |
| LEAP-Seq n confirming: | 496 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACACACAGGCTTCTCAG |
| Suggested primer 2: | GTGCATACGAGCAGTGGAGA |