| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239846 |
| Chromosome: | chromosome 1 |
| Location: | 3805160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024601 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCAGGAACGGTTCGGCTCCGTTACCTG |
| Internal bar code: | TCGTGGTGAAACTTCCCTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 98.125 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTGACCAGCGTTTTCTC |
| Suggested primer 2: | GTTTGGGCCTGTGAGTTCAT |