| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239848 |
| Chromosome: | chromosome 10 |
| Location: | 3419741 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444550 | SPP1A,SPPA1,SPP1 | Signal peptide peptidase; (1 of 1) K04773 - protease IV [EC:3.4.21.-] (sppA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACCGTCACCACTACCACCGCGACGGA |
| Internal bar code: | CCGCATGGCAGCGCAGCAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 99.8712 |
| LEAP-Seq n confirming: | 3878 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACAATGCTCTTGTACGC |
| Suggested primer 2: | AGAATGACGCCAGCAGAGTT |