| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239868 |
| Chromosome: | chromosome 3 |
| Location: | 3837278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170650 | (1 of 1) IPR001849//IPR011993 - Pleckstrin homology domain // Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATTCTTCCCGCATTCCAGCGGGAGGCG |
| Internal bar code: | GGGGTCCATTCCCTGTCAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 699 |
| LEAP-Seq percent confirming: | 99.3047 |
| LEAP-Seq n confirming: | 1571 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGTTGCATTGTGGGAC |
| Suggested primer 2: | TACACACACACCCGCTGATT |