Insertion junction: LMJ.RY0402.239868_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.239868
Chromosome:chromosome 3
Location:3837318
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g170650 (1 of 1) IPR001849//IPR011993 - Pleckstrin homology domain // Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAGACGAGCCGGACCAACCCTCGCCATGA
Internal bar code:GGTCTAATTTGTAACCGTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:672
LEAP-Seq percent confirming:99.6241
LEAP-Seq n confirming:265
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACACACACACCCGCTGATT
Suggested primer 2:AAGAGGTTGCATTGTGGGAC