| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239870 |
| Chromosome: | chromosome 12 |
| Location: | 9086532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542850 | ARD1 | (1 of 1) 1.13.11.54 - Acireductone dioxygenase (Fe(2+)-requiring) / E-2'; Aci-reductone dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGACACGGGTAATCATGTGGTTGCCTTG |
| Internal bar code: | AGTCGGTAAGGTCTTCGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 99.5731 |
| LEAP-Seq n confirming: | 1866 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCCAGGTGACTGTCTGT |
| Suggested primer 2: | TACGTACGCATTCGTTTCCA |