Insertion junction: LMJ.RY0402.239880_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.239880
Chromosome:chromosome 12
Location:7659963
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAGTATTAGCGCAGGAACAACCGCTTCGC
Internal bar code:TGTCCAACGACAGGAAGACGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:497
LEAP-Seq percent confirming:98.2315
LEAP-Seq n confirming:611
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGCACATTCCCTAATCA
Suggested primer 2:CTGGTCTGCTCTGGGTTCTC