Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.239882 |
Chromosome: | chromosome_12 |
Location: | 7085125 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g559400 | PDE12 | 3\',5\'-cyclic-nucleotide phosphodiesterase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTGCAACTTAGTGGTGCTCCTCTGCCAC |
Internal bar code: | TCCACCCCAGGTACGATCGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 548 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 375 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGACTTGGTGGGTTTCAA |
Suggested primer 2: | CGTGGGTTTCCTGTCATTCT |