| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239889 |
| Chromosome: | chromosome 4 |
| Location: | 341712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217909 | OPR124 | OctotricoPeptide Repeat protein 124 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCAGGAGGCTGCAGCGGCGGCGCTCG |
| Internal bar code: | ACTCGCGTTCCCACTCTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 73.0402 |
| LEAP-Seq n confirming: | 382 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTCGCTTTTTCCTTTCG |
| Suggested primer 2: | CTCCGAGCTCGTGTAACCTC |