Insertion junction: LMJ.RY0402.239889_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.239889
Chromosome:chromosome 4
Location:2957641
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAAAGCACTGATAATTGTGTGATGCTTG
Internal bar code:TGTGTCGCGTGTTTTTAAATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:98.791
LEAP-Seq n confirming:572
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGTTTTGTCCGTCAGG
Suggested primer 2:GTGTTTGGTTGCATGTTTGC