| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239901 |
| Chromosome: | chromosome_13 |
| Location: | 3736480 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589400 | PRL6 | Predicted extracellular protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTTGTTGGCTGCTAGAAGGAGCATGAAG |
| Internal bar code: | GTGTTGACCGCCGTGGCCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.6678 |
| LEAP-Seq n confirming: | 900 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGTGTACGACTTCACG |
| Suggested primer 2: | GAGGCACTAACTCCGTCTGC |