| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239950 |
| Chromosome: | chromosome 9 |
| Location: | 5517182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400516 | (1 of 6) K04710 - ceramide synthetase (CERS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAATGCAGGTCAGCAGGTGCCCAGGGGA |
| Internal bar code: | ACGGAGCTTAAATGCTCCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 96.7797 |
| LEAP-Seq n confirming: | 571 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGCTGCGAACTGAGATT |
| Suggested primer 2: | CGTGGTGTTTTTCCTGTCCT |