| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239956 |
| Chromosome: | chromosome 8 |
| Location: | 2406924 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g371052 | IRE1 | (1 of 1) PTHR12506//PTHR12506:SF25//PTHR13954//PTHR13954:SF6 - PROTEIN PHOSPHATASE RELATED // SUBFAMILY NOT NAMED // IRE1-RELATED // SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACGAAGAGGAGGCGTAGCCAGGTACAG |
| Internal bar code: | TTGTTGTCTTTCAGATATTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 95.5834 |
| LEAP-Seq n confirming: | 2597 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGTTGCGTAGCGTTGT |
| Suggested primer 2: | GGATTGTGGTTTCAGAGCGT |