| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239957 |
| Chromosome: | chromosome 13 |
| Location: | 4018694 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591073 | (1 of 2) PF00564 - PB1 domain (PB1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCGGCCGAGCCTCACGAACCCTCAGGT |
| Internal bar code: | TTCAGAGTGCTGTGCACACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 155 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGCAGTTGATCTCCAA |
| Suggested primer 2: | AACACCGAGTCCCTGTTGAG |