Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.239963 |
Chromosome: | chromosome_8 |
Location: | 1359236 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g364000 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCAGTATGTATTCACAACGGCTCCACATG |
Internal bar code: | TCGGAGGAGCTGCTAGGTCGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 149 |
LEAP-Seq percent confirming: | 98.5959 |
LEAP-Seq n confirming: | 3511 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGATTAGTGTCCACACGCT |
Suggested primer 2: | AGCGAGCTGCATCTACCATT |