| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239983 |
| Chromosome: | chromosome 6 |
| Location: | 2960576 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273100 | (1 of 3) PF03067 - Chitin binding domain (Chitin_bind_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCGTGCCGCCGTGGCGTCTGCAACGAC |
| Internal bar code: | GGCTGTCAGGAGTCAATGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 99.1794 |
| LEAP-Seq n confirming: | 1813 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGAGCTGCCAACATAA |
| Suggested primer 2: | TAAGAACGGTTGGCAGCTCT |