| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239999 |
| Chromosome: | chromosome 7 |
| Location: | 2609368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330150 | (1 of 7) IPR011335 - Restriction endonuclease type II-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGATTCGCATCAAACCTTTCCCTAAAT |
| Internal bar code: | ATACTATCGGAGAACCCAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.8102 |
| LEAP-Seq n confirming: | 526 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCGTGTCCCTCTTATGT |
| Suggested primer 2: | CAGACGTGGTATCACATGGC |