| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240018 |
| Chromosome: | chromosome 14 |
| Location: | 3509962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630871 | ZCP1 | Zn chaperone; (1 of 2) PTHR13748//PTHR13748:SF44 - COBW-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCTCTCTACCTGCCCCCCTCTCCGACC |
| Internal bar code: | GTTAAAAGGGACCGACCATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.4646 |
| LEAP-Seq n confirming: | 3901 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTGGCTTGCTTGCTATT |
| Suggested primer 2: | ACCAGGTCAATGTCTCGGTC |