| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240023 |
| Chromosome: | chromosome 14 |
| Location: | 2812880 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627411 | (1 of 2) PTHR13146//PTHR13146:SF0 - UNCHARACTERIZED // SOLUTE CARRIER FAMILY 35 MEMBER F6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAATGTGTAGCTCACAAGTCACAAGTC |
| Internal bar code: | GCGCAGGTCAGTGGTACGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 264 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCATGCATGACCACCAG |
| Suggested primer 2: | CTGGTGACCCAACGTCTTTT |