| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240034 |
| Chromosome: | chromosome 12 |
| Location: | 514684 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493150 | LTO1,CPLD41,VKE1 | Vitamin K epoxide reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTCATGGTGGCCTCTGTCCGCAAGAGT |
| Internal bar code: | ACCGACTCCCGTTTTTCGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.927 |
| LEAP-Seq n confirming: | 1369 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGAGGTGGAGAAGGAG |
| Suggested primer 2: | CTCCACGACCTCTGTCAAGC |