| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240037 |
| Chromosome: | chromosome_3 |
| Location: | 7881570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g202550 | ELG11 | Exostosin-like glycosyltransferase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCTGGGCTACAGCGACTGTGAGCCCCAAG |
| Internal bar code: | CGGACTCCCCCGGTTGTTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 99.2611 |
| LEAP-Seq n confirming: | 6851 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTTGACGCAGGAAGAAG |
| Suggested primer 2: | AAGGAGTAGGCGCAAAGACA |