| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240044 |
| Chromosome: | chromosome 12 |
| Location: | 7978274 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552150 | (1 of 1) PF00069//PF13185 - Protein kinase domain (Pkinase) // GAF domain (GAF_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGGTTTTGCGCGCCGGGCGAGGAATG |
| Internal bar code: | ATCGGTGGGGAAAAGCAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCAAATCGTGGTGAATG |
| Suggested primer 2: | CAGTTTGCTCCTCCTCCTTG |