| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240060 |
| Chromosome: | chromosome 7 |
| Location: | 1116641 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320200 | (1 of 1) PTHR23244:SF208 - F14M2.9 PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATGCGCGTCTAGAAGACCAGCCACCCC |
| Internal bar code: | TGCGATGAGCTAATAAGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 98.6989 |
| LEAP-Seq n confirming: | 531 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCGTGAAAGAAGCCTGT |
| Suggested primer 2: | ATGGGAGCGTGTAAGAGGTG |