Insertion junction: LMJ.RY0402.240066_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.240066
Chromosome:chromosome 17
Location:6123054
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g741500 KIL7 Putative kinesin-like motor protein; (1 of 1) 3.4.21.72//3.6.4.4 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease // Plus-end-directed kinesin ATPase / Kinesin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCAAGCACGTGCGCTAACCGGGTGAGGA
Internal bar code:GGCGGATGGATTGCGGGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:631
LEAP-Seq percent confirming:97.6562
LEAP-Seq n confirming:125
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCAGGTTGGTGTACTTTT
Suggested primer 2:GGAGTAGAAGCAATGTCGCC