| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240071 |
| Chromosome: | chromosome 12 |
| Location: | 1561374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTTTGACTGGCGGGATGGAGGCGAGGC |
| Internal bar code: | TGCGGGTCGTAGGCACGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.7122 |
| LEAP-Seq n confirming: | 693 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACAGAGCTCCGTGAACA |
| Suggested primer 2: | GCTGAAAGCTCATCAGGTCC |