Insertion junction: LMJ.RY0402.240080_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.240080
Chromosome:chromosome 12
Location:7518003
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g555950 DHC11 Axonemal Dynein Heavy Chain 11; (1 of 1) PTHR10676//PTHR10676:SF265 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGCGCGCCGTCAAGGCCGCGGTGCTCGA
Internal bar code:GTCGAACCGGTGTCTGTGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:186
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTCGTGGTCAGGTACG
Suggested primer 2:ACAGTGTCACAAGGAAGGGC