| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240080 |
| Chromosome: | chromosome 12 |
| Location: | 7518025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555950 | DHC11 | Axonemal Dynein Heavy Chain 11; (1 of 1) PTHR10676//PTHR10676:SF265 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTCGGGCGTGAGGCGCGCCAGCGTCT |
| Internal bar code: | GATCTTTACCACGGTGTGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.7027 |
| LEAP-Seq n confirming: | 2012 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTTCGTGGTCAGGTACG |
| Suggested primer 2: | ACAGTGTCACAAGGAAGGGC |