| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240086 |
| Chromosome: | chromosome 17 |
| Location: | 2445332 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715800 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGATGAGCACGCTCTCGGTCTTGGCGG |
| Internal bar code: | TCACAGTAGTTGCTTGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 99.1348 |
| LEAP-Seq n confirming: | 2750 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGCCTATTGCTAGTCCT |
| Suggested primer 2: | TGATGCGCGGTACTTACTTG |