| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240086 |
| Chromosome: | chromosome 17 |
| Location: | 2445332 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715800 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGACTACTCGGCATGCGGGTGCGTGG |
| Internal bar code: | GTCGATTTACGGTGGAACGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 99.7814 |
| LEAP-Seq n confirming: | 4564 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCGCGGTACTTACTTG |
| Suggested primer 2: | TTGCGCCTATTGCTAGTCCT |