Insertion junction: LMJ.RY0402.240136_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.240136
Chromosome:chromosome 3
Location:7971326
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201950 (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCAAGCCGCCCAGCCCGACCCCAAGCC
Internal bar code:CGCGCGGTTGTGGGTGGACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:76.5568
LEAP-Seq n confirming:209
LEAP-Seq n nonconfirming:64
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGATCTCGTGTACCTGGC
Suggested primer 2:GATCTTCGCATTCAAGCACA