| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240220 |
| Chromosome: | chromosome 2 |
| Location: | 380449 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075900 | STK9,STPK9,SNRK2C | (1 of 1) IPR000719//IPR001245//IPR002290//IPR004223//IPR011009//IPR020635 - Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Vitamin B12-dependent methionine synthase, activation domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain; snRK1 family in Chlamydomonas, subgroup 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGCGGCAGGCGTGCGAGTTGACAGTTG |
| Internal bar code: | GGAGACTATCCGATGTGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.6528 |
| LEAP-Seq n confirming: | 1435 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATGTGTGCAGATTGCGGA |
| Suggested primer 2: | CATGTACCCAAAACACGCAG |