Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.240226 |
Chromosome: | chromosome_3 |
Location: | 5077043 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g181150 | DLL1,ROC106,FLA14,RSP22,LC8 | Dynein arm light chain 8, LC8 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTAGCAAAATGGCGTCGGGTAGCTCCAAGG |
Internal bar code: | GACGGCAGCCACAGGTCGTGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 936 |
LEAP-Seq percent confirming: | 98.8543 |
LEAP-Seq n confirming: | 604 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAATGTATTTCGACCCTGCG |
Suggested primer 2: | CATCTTCCTCCCCTTCCTTC |