Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.240226 |
Chromosome: | chromosome_3 |
Location: | 5428284 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g185000 | BGS2,GTR8 | 1,3-beta-D-glucan synthase | antisense | 5'UTR_intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAAAGGTCGTGTCAGGCAGTGGCGAAAGGC |
Internal bar code: | TGGGTCCAACACGTCCGTGTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 474 |
LEAP-Seq percent confirming: | 97.7053 |
LEAP-Seq n confirming: | 809 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGACAGCCACAAGGCTAGAG |
Suggested primer 2: | CACAACCATGCCTCAACAAC |