Insertion junction: LMJ.RY0402.240265_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.240265
Chromosome:chromosome 2
Location:4984407
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g104150 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAGGGCGAGTCGCTGCGCGCTGAAGTGG
Internal bar code:CCGTTCGAATTCGTCTATACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:674
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTACCCCTTGCCCAGTTG
Suggested primer 2:CGCTGTTCCTCTAGGGTCTG