| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240281 |
| Chromosome: | chromosome 10 |
| Location: | 4877220 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454400 | (1 of 1) K10632 - BRCA1-associated protein (BRAP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGGGCCGGACTCTCTGCCTGTAATA |
| Internal bar code: | CTCGCGGGCACATCGTGCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 99.6894 |
| LEAP-Seq n confirming: | 321 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAACCAAGCAAGCATACC |
| Suggested primer 2: | CGTGGTGTACGACCTCAATG |