| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.240287 |
| Chromosome: | chromosome_11 |
| Location: | 874036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467653 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCTCTAGCCGGTAGTTGTTAGCAAGCCAG |
| Internal bar code: | GCGACGATCGGTAGCGGGTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 99.3821 |
| LEAP-Seq n confirming: | 965 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTACGGTGGAGGCTACGG |
| Suggested primer 2: | TCGTAACAACCGAGCAAGTG |