| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240308 |
| Chromosome: | chromosome 7 |
| Location: | 5414666 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350350 | FAP217 | Coiled-Coil Flagellar Associated Protein 217 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAGTCTCACCTTAAAAGCCCTGCGCTC |
| Internal bar code: | TATCTAATTCCTTGACCGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCATAACCACGCGACAT |
| Suggested primer 2: | CCAGTACTCCTCCTGCTTGC |