| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240339 |
| Chromosome: | chromosome 4 |
| Location: | 1185911 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215600 | (1 of 1) PTHR32183:SF6 - THIOCYANATE METHYLTRANSFERASE 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGAAAGGGCAAACCTGTTGCTGCAGAGT |
| Internal bar code: | GGTGGCGACTGTTAGTCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 98.983 |
| LEAP-Seq n confirming: | 292 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGATGCCCAGTGCAGTA |
| Suggested primer 2: | CAGCGCACTCTCATGCTTAG |