| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240363 |
| Chromosome: | chromosome 10 |
| Location: | 5179490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456700 | CASC3,EJC1 | (1 of 1) PF09405 - CASC3/Barentsz eIF4AIII binding (Btz); Exon Junction Complex component | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACAACACGCACTATGGACATAAGGAAC |
| Internal bar code: | CGCCTCCTGATGCGTTATCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.7681 |
| LEAP-Seq n confirming: | 4732 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGTTTTGTAGGGGCACT |
| Suggested primer 2: | TCAATTGCCAAGCTTCACTG |