| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240368 |
| Chromosome: | chromosome 6 |
| Location: | 7262745 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298350 | FAP224 | Flagellar Associated Protein 224; (1 of 1) PTHR14208//PTHR14208:SF2 - BASIC LEUCINE ZIPPER AND W2 DOMAIN-CONTAINING PROTEIN // PROTEIN KRASAVIETZ | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATACTCGTGCGCTTGCAGGCCGCTGGCCT |
| Internal bar code: | TAATCTTGCCAGTGGTCTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGACGTGGCCCTTACAT |
| Suggested primer 2: | CACACCTGGATGGTGTTCAG |